COMPARISON OF POLYETHYLENE-GLYCOL PRECIPITATION AND ULTRACENTRIFUGATION FOR RECOVERY OF CYTOMEGALOVIRUS FROM URINE PRIOR TO DETECTION OF DNA BY DOT-BLOT HYBRIDIZATION

被引：16

作者：

KIMPTON, CP ^{[1
]}

CORBITT, G ^{[1
]}

MORRIS, DJ ^{[1
]}

机构：

[1] UNIV MANCHESTER, SCH MED,DEPT PATHOL SCI,DIV VIROL,STOPFORD BLDG, OXFORD RD, MANCHESTER M13 9PT, LANCS, ENGLAND

来源：

JOURNAL OF VIROLOGICAL METHODS | 1990年 / 28卷 / 02期

关键词：

Cytomegalovirus; Polyethylene glycol; Recovery; Ultracentrifugation; Urine;

D O I：

10.1016/0166-0934(90)90028-E

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Polyethylene glycol (PEG) precipitation provided a useful alternative to ultracentrifugation for recovering cytomegalovirus (CMV) from clinical specimens prior to DNA hybridisation studies. The conditions for use of PEG are described. In a study of 61 urine samples from patients suspected of CMV infection 18 yielded a positive DNA hybridisation result. Fifteen were positive after both concentration procedures, two after PEG precipitation only and one after ultracentrifugation only. The DNA hybridisation results are discussed in the light of the results of virus isolation by cell culture. © 1990.

引用

页码：141 / 145

页数：5

共 11 条

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