A NEW METHOD OF SYNTHESIS OF FLUORESCENTLY LABELED OLIGONUCLEOTIDES AND THEIR APPLICATION IN DNA SEQUENCING

被引：14

作者：

MARKIEWICZ, WT ^{[1
]}

GROGER, G ^{[1
]}

ROSCH, R ^{[1
]}

ZEBROWSKA, A ^{[1
]}

SELIGER, H ^{[1
]}

机构：

[1] UNIV ULM,SEKT POLYMERE,W-7900 ULM,GERMANY

来源：

NUCLEOSIDES & NUCLEOTIDES | 1992年 / 11卷 / 10期

关键词：

D O I：

10.1080/07328319208017817

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new approach to the chemical synthesis of oligonucleotides bearing reporter functional groups based on the modification of cytosine residues during the final deprotection step is reported. The application of the fluorescently labelled primer for the automated DNA sequencing is shown.

引用

收藏

页码：1703 / 1711

页数：9

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