RIBOSOME-MESSENGER RECOGNITION IN THE ABSENCE OF THE SHINE-DALGARNO INTERACTIONS

被引：92

作者：

TZAREVA, NV

MAKHNO, VI

BONI, IV

机构：

[1] RUSSIAN ACAD SCI, MM SHEMYAKIN & YA OVCHINNIKOV INST BIOORGAN CHEM, 117871 MOSCOW, RUSSIA

[2] RUSSIAN ACAD SCI, PETERSBURG NUCL PHYS INST, 188350 GATCHINA, RUSSIA

来源：

FEBS LETTERS | 1994年 / 337卷 / 02期

关键词：

TRANSLATIONAL INITIATION; RNA-PROTEIN INTERACTION; PLANT VIRAL RNA LEADER; PROTEIN S1; TOEPRINTING; FOOTPRINTING;

D O I：

10.1016/0014-5793(94)80271-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In an attempt to understand how Escherichia coli ribosomes recognize the initiator codon on mRNAs lacking the Shine-Dalgarno (SD) sequence, we have studied 30S initiation complex formation in extension inhibition (toeprinting) experiments using (-SD)mRNAs which are known to be reliably translated in E. coli: the plant viral messenger A1MV RNA 4 and two chimaeric mRNAs coding for beta-glucuronidase (GUS) and bearing the 5'-untranslated sequence of TMV RNA (Omega) or the Omega-derived sequence (CAA)(n) as 5'-leaders. Ribosomal protein S1 and IF3 have been found to be indispensable for translational initiation. Protein S1 appears to be a key recognition element. S1 binds to sequences within the leaders of (-SD)mRNAs thus providing their affinity to E. coli ribosomes.

引用

收藏

页码：189 / 194

页数：6

相关论文

共 27 条

[1] RIBOSOMAL-PROTEIN S1 ASSOCIATES WITH ESCHERICHIA-COLI RIBOSOMAL 30-S SUBUNIT BY MEANS OF PROTEIN-PROTEIN INTERACTIONS [J].

BONI, IV ;

ZLATKIN, IV ;

BUDOWSKY, EI .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1982, 121 (02) :371-376

[2] RIBOSOME-MESSENGER RECOGNITION - MESSENGER-RNA TARGET SITES FOR RIBOSOMAL-PROTEIN S1 [J].

BONI, IV ;

ISAEVA, DM ;

MUSYCHENKO, ML ;

TZAREVA, NV .

NUCLEIC ACIDS RESEARCH, 1991, 19 (01) :155-162

[3] COMPLETE NUCLEOTIDE-SEQUENCE OF ALFALFA MOSAIC-VIRUS RNA-4 [J].

BREDERODE, FT ;

KOPERZWARTHOFF, EC ;

BOL, JF .

NUCLEIC ACIDS RESEARCH, 1980, 8 (10) :2213-2223

[4] SELECTION OF THE MESSENGER-RNA TRANSLATION INITIATION REGION BY ESCHERICHIA-COLI RIBOSOMES [J].

CALOGERO, RA ;

PON, CL ;

CANONACO, MA ;

GUALERZI, CO .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (17) :6427-6431

[5] ALTERNATIVE OCCUPANCY OF A DUAL RIBOSOMAL-BINDING SITE BY MESSENGER-RNA AFFECTED BY TRANSLATION INITIATION-FACTORS [J].

CANONACO, MA ;

GUALERZI, CO ;

PON, CL .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1989, 182 (03) :501-506

[6] TRANSLATION BY ESCHERICHIA-COLI RIBOSOMES OF ALFALFA MOSAIC-VIRUS RNA 4 CAN BE INITIATED AT 2 SITES ON THE MONOCISTRONIC MESSAGE [J].

CASTEL, A ;

KRAAL, B ;

KONIECZNY, A ;

BOSCH, L .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1979, 101 (01) :123-133

[7]

DESMIT MH, 1990, PROG NUCLEIC ACID RE, V38, P1

[8] THE EFFECT OF RIBOSOMAL PROTEIN-S1 FROM ESCHERICHIA-COLI AND MICROCOCCUS-LUTEUS ON PROTEIN-SYNTHESIS INVITRO BY ESCHERICHIA-COLI AND BACILLUS-SUBTILIS [J].

FARWELL, MA ;

ROBERTS, MW ;

RABINOWITZ, JC .

MOLECULAR MICROBIOLOGY, 1992, 6 (22) :3375-3383

[9] A TRANSLATIONAL ENHANCER DERIVED FROM TOBACCO MOSAIC-VIRUS IS FUNCTIONALLY EQUIVALENT TO A SHINE-DALGARNO SEQUENCE [J].

GALLIE, DR ;

KADO, CI .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (01) :129-132

[10] MUTATIONAL ANALYSIS OF THE TOBACCO MOSAIC-VIRUS 5'-LEADER FOR ALTERED ABILITY TO ENHANCE TRANSLATION [J].

GALLIE, DR ;

SLEAT, DE ;

WATTS, JW ;

TURNER, PC ;

WILSON, TMA .

NUCLEIC ACIDS RESEARCH, 1988, 16 (03) :883-893