HPLC SEPARATION AND QUANTITATION OF PHYTIC ACID AND SOME INOSITOL PHOSPHATES IN FOODS - PROBLEMS AND SOLUTIONS

被引:132
作者
LEHRFELD, J
机构
[1] National Center for Agricultural Utilization Research, Food Physical Chemistry, Agricultural Research Service, U.S. Department of Agriculture, Peoria, Illinois 61604
关键词
HPLC; PHYTIC ACID; INOSITOL PHOSPHATES; ION-PAIR CHROMATOGRAPHY;
D O I
10.1021/jf00048a015
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
A macroporous polymer column was used to resolve a mixture of inositol hexakis-, pentakis-, tetrakis-, and trisphosphates within 5-9 min. Optimized eluting systems based on acetonitrile or methanol had improved resolution and reduced chromatographic run times. The silica-based anion exchange column used in the analysis of cereal samples was used to prepare small amounts of pure inositol pentakis-, tetrakis-, and trisphosphates for calibration standards. A number of potential analytical problems are discussed, and solutions are proposed.
引用
收藏
页码:2726 / 2731
页数:6
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