A MULTIPLEXED SINGLE-PHOTON INSTRUMENT FOR ROUTINE MEASUREMENT OF TIME-RESOLVED FLUORESCENCE ANISOTROPY

被引：24

作者：

BIRCH, DJS ^{[1
]}

HOLMES, AS ^{[1
]}

GILCHRIST, JR ^{[1
]}

IMHOF, RE ^{[1
]}

ALALAWI, SM ^{[1
]}

NADOLSKI, B ^{[1
]}

机构：

[1] EDINBURGH INSTRUMENTS LTD,EDINBURGH EH14 4AP,SCOTLAND

来源：

JOURNAL OF PHYSICS E-SCIENTIFIC INSTRUMENTS | 1987年 / 20卷 / 04期

关键词：

OPTICAL INSTRUMENTS - Design;

D O I：

10.1088/0022-3735/20/4/031

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

We report a new type of instrument, the Edinburgh Instruments Model 299T differential fluorometer, for the measurement of the decay of fluorescence anisotropy. The instrument incorporates three multiplexed detection channels for simultaneous measurement of two polarized fluorescence decays and the excitation pulse, using time-correlated single-photon counting. This arrangement corrects automatically for temporal and intensity fluctuations of the spark source. A study of the probe 1,6-diphenyl-1,3,5-hexatriene (DPH) in a white oil reveals that the instrument can measure rotational correlation times with subnanosecond time resolution in a third of the time of conventional methods.

引用

页码：471 / 473

页数：3

共 14 条

[1] COAXIAL NANOSECOND FLASHLAMP
BIRCH, DJS
IMHOF, RE
[J]. REVIEW OF SCIENTIFIC INSTRUMENTS, 1981, 52 (08) : 1206 - 1212
[2] PULSE FLUOROMETRY USING SIMULTANEOUS ACQUISITION OF FLUORESCENCE AND EXCITATION
BIRCH, DJS
IMHOF, RE
DUTCH, A
[J]. REVIEW OF SCIENTIFIC INSTRUMENTS, 1984, 55 (08) : 1255 - 1264
[3] BIRCH DJS, 1985, ANAL INSTRUM, V14, P293
[4] DIFFERENTIAL PULSE FLUOROMETRY USING MATCHED PHOTOMULTIPLIERS - A NEW METHOD OF MEASURING FLUORESCENCE LIFETIMES
BIRCH, DJS
IMHOF, RE
DUTCH, A
[J]. JOURNAL OF PHYSICS E-SCIENTIFIC INSTRUMENTS, 1984, 17 (05): : 417 - 418
[5] BIRCH DJS, 1986, EDINBURGH INSTRUMENT, V1
[6] FLUORESCENCE OF DIPHENYL-POLYENES AND RETINOL-POLYENES
BIRKS, JB
BIRCH, DJS
[J]. CHEMICAL PHYSICS LETTERS, 1975, 31 (03) : 608 - 610
[7] DALE RE, 1977, J BIOL CHEM, V252, P7500
[8] DALE RE, 1983, TIME RESOLVED FLUORE, P555
[9] RETRO-DIFFRACTED LIGHT IN FLUORESCENCE SPECTROMETERS
DUTCH, A
BIRCH, DJS
IMHOF, RE
[J]. CHEMICAL PHYSICS LETTERS, 1986, 125 (01) : 57 - 63
[10] FLUORESCENCE DEPOLARIZATION STUDIES ON FLEXIBILITY OF MYOSIN ROD
HARVEY, SC
CHEUNG, HC
[J]. BIOCHEMISTRY, 1977, 16 (24) : 5181 - 5187

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