MEASUREMENT OF COPY NUMBER USING HPLC

被引：4

作者：

COPPELLA, SJ ^{[1
]}

ACHESON, CM ^{[1
]}

DHURJATI, P ^{[1
]}

机构：

[1] UNIV DELAWARE,DEPT CHEM ENGN,NEWARK,DE 19716

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1987年 / 29卷 / 05期

关键词：

BIOLOGICAL MATERIALS - Characterization - CHROMATOGRAPHIC ANALYSIS - High Pressure Liquid - GENETIC ENGINEERING - NUCLEIC ACIDS - Chromatographic Analysis;

D O I：

10.1002/bit.260290514

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Copy number, the number of expression vectors per host genome, is a key variable in recombinant microbial system study. A quantitative method of monitoring copy number, which is relatively straightforward and quick, is presented here. Cells are first sonicated and then centrifuged to remove debris. The supernatant was filtered to further remove debris and then injected into a Nucleogen 4000 DEAE HPLC system. The ratio of plasmid DNA peak to the chromosomal DNA peak correlated well with the expected variation in the copy numbers of the temperature sensitive recombinant system that was studied.

引用

页码：646 / 647

页数：2