RAPID KINETICS OF AGONIST-EVOKED CHANGES IN CYTOSOLIC FREE CA-2+ CONCENTRATION IN FURA-2-LOADED HUMAN NEUTROPHILS

The initial kinetics of agonist-evoked rises in the cytosolic Ca2+ concentration (Ca2+](i) were investigated in fura-2-loaded human neutrophils by stopped-flow fluorimetry. The rises in [Ca2+](i) evoked by chemotactic peptide (fMet-Leu-Phe), platelet-activating factor and ADP all lagged behind agonist addition by 1-1.3 s. Lag times were not significantly different in the presence and in the absence of external Ca2+. Stimulation of the cells in the presence of extracellular Mn2+ resulted in a quench of fluorescence with a similar lag time to [Ca2+](i) rise. The delay in onset of the rise in [Ca2+](i) evoked by fMet-Leu-Phe was dependent on concentration, becoming longer at lower concentrations of agonist. These results indicate that both the agonist-evoked discharge of the intracellular Ca2+ stores and the generation of bivalent-cation influx lag behind agonist-receptor binding in neutrophils. Both pathways thus appear to be mediated by indirect mechanisms, rather than by a directly coupled process such as a receptor-operated channel. The temporal coincidence of the onset of store discharge with the commencement of bivalent-cation influx suggests that the two events may be causally linked.