REGULARITIES OF INSITU NICK TRANSLATION LABELING PATTERN OF CEREAL METAPHASE CHROMOSOMES

被引:2
作者
KOST, MV
BOLSHEVA, NL
BADAEV, NS
ZELENIN, AV
机构
关键词
WHEAT; MODIFIED NICK TRANSLATION; RYE;
D O I
10.1139/g91-020
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A method of nick translation in situ that makes it possible to detect regions hypersensitive to DNase I in metaphase chromosomes has been modified for plants. Such regions have a peculiar structural conformation typical of transcriptionally active chromatin. The distribution of transcriptionally active regions labeled with [H-3]TTP along the length of individual chromosomes of rye and B genome of wheat was studied. The following regularities of this distribution were found: a weak labeling of heterochromatic regions; an increase in the intensity of labeling in regions located at the border of euchromatin and heterochromatin and in the nucleolar organizer regions. Each chromosome of the B and R genomes has its own labeling pattern, but some similarities in the labeling pattern in all chromosomes have been revealed. Such similarities are characteristic of homoeologous chromosomes.
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页码:131 / 138
页数:8
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