IMPROVING THE ENANTIOSELECTIVITY OF THE CANDIDA-CYLINDRACEA LIPASE VIA CHEMICAL MODIFICATION

被引：43

作者：

GU, QM ^{[1
]}

SIH, CJ ^{[1
]}

机构：

[1] UNIV WISCONSIN, SCH PHARM, MADISON, WI 53706 USA

来源：

BIOCATALYSIS | 1992年 / 6卷 / 02期

基金：

美国国家卫生研究院;

关键词：

ENANTIOSELECTIVITY; LIPASE; CANDIDA-CYLINDRACEA; CHEMICAL MODIFICATION; TETRANITROMETHANE; TYROSYL RESIDUES; ENANTIOSELECTIVE HYDROLYSIS;

D O I：

10.3109/10242429209014887

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The lipase of Candida cylindracea (B-form) was treated with the classical chemoselective reagents with a view to modifying the specific amino acid residue(s) in the protein. Nitration of the tyrosyl residues of this enzyme was achieved using an excess of tetranitromethane (TNM). This chemically modified TNM-lipase showed a remarkable improvement in enantioselectivity towards the hydrolysis of a series of aryloxypropionic and arylpropionic esters. © 1992 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

引用

页码：115 / 126

页数：12

共 19 条

[1] MECHANISM OF PANCREATIC LIPASE ACTION .2. CATALYTIC PROPERTIES OF MODIFIED LIPASES [J].

CHAPUS, C ;

SEMERIVA, M .

BIOCHEMISTRY, 1976, 15 (23) :4988-4991

[2] QUANTITATIVE-ANALYSES OF BIOCHEMICAL KINETIC RESOLUTIONS OF ENANTIOMERS [J].

CHEN, CS ;

FUJIMOTO, Y ;

GIRDAUKAS, G ;

SIH, CJ .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1982, 104 (25) :7294-7299

[3] REGRESSION-ANALYSIS OF NONLINEAR ARRHENIUS PLOTS - AN EMPIRICAL-MODEL AND A COMPUTER-PROGRAM [J].

DUGGLEBY, RG .

COMPUTERS IN BIOLOGY AND MEDICINE, 1984, 14 (04) :447-455

[4] CHARACTERIZATION OF THE SERINE REACTING WITH DIETHYL RHO-NITROPHENYL PHOSPHATE IN PORCINE PANCREATIC LIPASE [J].

GUIDONI, A ;

BENKOUKA, F ;

DECARO, J ;

ROVERY, M .

BIOCHIMICA ET BIOPHYSICA ACTA, 1981, 660 (01) :148-150

[5]

KAISER ET, 1985, ANNU REV BIOCHEM, V54, P565

[6] EFFECTS OF CHEMICAL MODIFICATION OF AMINO-ACID RESIDUES ON THE ACTIVITIES OF LIPASE FROM CANDIDA-CYLINDRACEA [J].

KAWASE, M ;

TANAKA, A .

ENZYME AND MICROBIAL TECHNOLOGY, 1989, 11 (01) :44-48

[7] MOLECULAR-CLONING AND NUCLEOTIDE-SEQUENCE OF THE LIPASE GENE FROM PSEUDOMONAS-FRAGI [J].

KUGIMIYA, W ;

OTANI, Y ;

HASHIMOTO, Y ;

TAKAGI, Y .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1986, 141 (01) :185-190

[8]

Means G.E., 1971, CHEM MODIFICATION PR

[9]

MUDERHWA JM, 1986, APPL MICROBIOL BIOT, V23, P348

[10] HYDROGEN ION EQUILIBRIA AND CHEMICAL MODIFICATION OF LYSINE AND TYROSINE RESIDUES IN BOVINE CARBONIC ANHYDRASE B [J].

NILSSON, A ;

LINDSKOG, S .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1967, 2 (03) :309-+

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