PCR AMPLIFICATION OF MICRODISSECTED WHEAT CHROMOSOME ARMS IN A SIMPLE SINGLE TUBE REACTION

被引：41

作者：

ALBANI, D ^{[1
]}

COTE, MJ ^{[1
]}

ARMSTRONG, KC ^{[1
]}

CHEN, QF ^{[1
]}

SEGAL, A ^{[1
]}

ROBERT, LS ^{[1
]}

机构：

[1] AGR CANADA,RES BRANCH,PLANT RES CTR,KW NEATBY BLDG,CENT EXPTL FARM,OTTAWA K1A 0C6,ONTARIO,CANADA

来源：

PLANT JOURNAL | 1993年 / 4卷 / 05期

关键词：

D O I：

10.1046/j.1365-313X.1993.04050899.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A novel method for the adaptor-mediated PCR amplification of microdissected chromosome arms is described. This simple and versatile protocol eliminates the need for enzymatic micromanipulation in nanoliter volumes and permits the efficient amplification of as little as two wheat chromosome arms in a 'single tube' reaction.

引用

页码：899 / 903

页数：5

共 15 条

[1] UNMETHYLATED CPG ISLANDS ASSOCIATED WITH GENES IN HIGHER-PLANT DNA
ANTEQUERA, F
BIRD, AP
[J]. EMBO JOURNAL, 1988, 7 (08) : 2295 - 2299
[2] HPAII LIBRARY INDICATES METHYLATION-FREE ISLANDS IN WHEAT AND BARLEY
CHEUNG, WY
MOORE, G
MONEY, TA
GALE, MD
[J]. THEORETICAL AND APPLIED GENETICS, 1992, 84 (5-6) : 739 - 746
[3] THE MOLECULAR CHARACTERIZATION AND ORGANIZATION OF PLANT CHROMOSOMAL DNA-SEQUENCES
FLAVELL, R
[J]. ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1980, 31 : 569 - 596
[4] MOLECULAR-CLONING OF DNA FROM SPECIFIC CHROMOSOMAL REGIONS BY MICRODISSECTION AND SEQUENCE-INDEPENDENT AMPLIFICATION OF DNA
JOHNSON, DH
[J]. GENOMICS, 1990, 6 (02) : 243 - 251
[5] A DNA LIBRARY FROM AN INDIVIDUAL BETA-PATELLARIS CHROMOSOME CONFERRING NEMATODE RESISTANCE OBTAINED BY MICRODISSECTION OF MEIOTIC METAPHASE CHROMOSOMES
JUNG, C
CLAUSSEN, U
HORSTHEMKE, B
FISCHER, F
HERRMANN, RG
[J]. PLANT MOLECULAR BIOLOGY, 1992, 20 (03) : 503 - 511
[6] CLONING DEFINED REGIONS OF THE HUMAN GENOME BY MICRODISSECTION OF BANDED CHROMOSOMES AND ENZYMATIC AMPLIFICATION
LUDECKE, HJ
SENGER, G
CLAUSSEN, U
HORSTHEMKE, B
[J]. NATURE, 1989, 338 (6213) : 348 - 350
[7] MOLECULAR CLONES OF THE MOUSE T COMPLEX DERIVED FROM MICRODISSECTED METAPHASE CHROMOSOMES
ROHME, D
FOX, H
HERRMANN, B
FRISCHAUF, AM
EDSTROM, JE
MAINS, P
SILVER, LM
LEHRACH, H
[J]. CELL, 1984, 36 (03) : 783 - 788
[8] Sandery M.J., 1991, PLANT MOL BIOL REP, V9, P21, DOI DOI 10.1007/BF02669286
[9] PCR AMPLIFICATION OF DNA MICRODISSECTED FROM A SINGLE POLYTENE CHROMOSOME BAND - A COMPARISON WITH CONVENTIONAL MICROCLONING
SAUNDERS, RDC
GLOVER, DM
ASHBURNER, M
SIDENKIAMOS, I
LOUIS, C
MONASTIRIOTI, M
SAVAKIS, C
KAFATOS, F
[J]. NUCLEIC ACIDS RESEARCH, 1989, 17 (22) : 9027 - 9037
[10] MICRO-DISSECTION AND CLONING OF DNA FROM A SPECIFIC REGION OF DROSOPHILA-MELANOGASTER POLYTENE CHROMOSOMES
SCALENGHE, F
TURCO, E
EDSTROM, JE
PIRROTTA, V
MELLI, M
[J]. CHROMOSOMA, 1981, 82 (02) : 205 - 216

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