PLATINUM ANTICANCER DRUG-BINDING TO DNA DETECTED BY THICKNESS-SHEAR-MODE ACOUSTIC-WAVE SENSOR

被引：75

作者：

SU, HB ^{[1
]}

WILLIAMS, P ^{[1
]}

THOMPSON, M ^{[1
]}

机构：

[1] UNIV TORONTO,DEPT CHEM,TORONTO,ON M5S 1A1,CANADA

来源：

ANALYTICAL CHEMISTRY | 1995年 / 67卷 / 05期

关键词：

D O I：

10.1021/ac00101a033

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Nucleic acid has been attached to the electrodes of thickness-shear-mode acoustic wave devices to produce a biosensor for platinum-based drugs. The decreases in series resonant frequency for interactions of DNA with both cis- and transplatin are indicative of two distinct kinetic processes, The results of a kinetic analysis are interpreted in terms of nucleic acid binding of the hydrolysis products of the two drugs. Concentration-dependent decreases of series resonant frequency show that the limit of detection for the drugs is approximately 10(-7) M. Motional resistance changes for nucleic acid-drug interactions also convey information regarding the chemistry of the macromolecules at the interface.

引用

页码：1010 / 1013

页数：4

共 17 条

[1] PT-195 NMR KINETIC AND MECHANISTIC STUDIES OF CIS-DIAMMINEDICHLOROPLATINUM AND TRANS-DIAMMINEDICHLOROPLATINUM(II) BINDING TO DNA [J].