ISOLATION AND CHARACTERIZATION OF THE ZWF1 GENE OF SACCHAROMYCES-CEREVISIAE, ENCODING GLUCOSE-6-PHOSPHATE-DEHYDROGENASE

被引:148
作者
NOGAE, I [1 ]
JOHNSTON, M [1 ]
机构
[1] WASHINGTON UNIV,SCH MED,DEPT GENET,BOX 8232,660 S EUCLID ST,ST LOUIS,MO 63110
关键词
glucose repression; pentose phosphate pathway; polymerase chain reaction; Recombinant DNA; yeast;
D O I
10.1016/0378-1119(90)90248-P
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Glucose-6-phosphate dehydrogenase (G6PD) catalyzes the first step of the pentose phosphate pathway, a reaction that generates NADPH. We have isolated ZWF1 the Saccharomyces cerevisiae gene that encodes G6PD, and identified its transcript and transcription start point. Expression of ZWF1 appears not to be regulated, consistent with its 'housekeeping' role. Null mutants lacking G6PD appear to grow normally, but are more sensitive than wild type to oxidizing agents that presumably reduce the level of NADPH. This suggests that G6PD has a major role in NADPH production in yeast. Regulation of GALI expression appears normal in zwfl mutants, suggesting that the pentose phosphate pathway is not involved in glucose repression. The predicted amino acid sequence of yeast G6PD is highly similar to the sequence of the Drosophila, human, and rat enzymes, except near its N terminus, where the yeast and Drosophila sequences diverge from that of human and rat. © 1990.
引用
收藏
页码:161 / 169
页数:9
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