AGROBACTERIUM-RHIZOGENES-MEDIATED TRANSFORMATION OF ECHINACEA-PURPUREA

被引：41

作者：

TRYPSTEEN, M

VANLIJSEBETTENS, M

VANSEVEREN, R

VANMONTAGU, M

机构：

[1] STATE UNIV GHENT,GENET LAB,LEDEGANCKSTR 35,B-9000 GHENT,BELGIUM

[2] STATE UNIV GHENT,PHARMACOGNOSY & PHYTOCHEM LAB,B-9000 GHENT,BELGIUM

来源：

PLANT CELL REPORTS | 1991年 / 10卷 / 02期

关键词：

AGROBACTERIUM; RHIZOGENES ALKAMIDES ECHINACEA-PURPUREA; TRANSFORMATION;

D O I：

10.1007/BF00236463

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Echinacea purpurea seedlings were inoculated with several Agrobacterium rhizogenes strains in order to obtain hairy roots. Infection with A. rhizogenes strains LMG63 and LMG150 resulted in callus formation. Upon infection with strains ATCC 15834 and R1601 hairy roots were obtained. Opine detection confirmed transformation of E. purpurea. Comparative HPLC fingerprint analysis of the alkamides from natural plant source, control tissues, and transformed callus and roots indicated that transformed callus and hairy roots might be a promising source for continuous and standardized production of the dodeca-2E,4E,8Z,10E/Z-tetraenoic acid isobutylamide and related amides.

引用

页码：85 / 89

页数：5

共 23 条

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