A NOVEL AT-RICH TANDEM REPEAT OF BRASSICA NIGRA

被引：29

作者：

GUPTA, V ^{[1
]}

JAGANNATHAN, V ^{[1
]}

LAKSHMIKUMARAN, MS ^{[1
]}

机构：

[1] TATA ENERGY RES INST,DIV BIOTECHNOL,90 JOR BAGH,NEW DELHI 110003,INDIA

来源：

PLANT SCIENCE | 1990年 / 68卷 / 02期

关键词：

Brassica; nucleotide sequence; repetitive DNA; tandem repeat;

D O I：

10.1016/0168-9452(90)90228-G

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A family of repeated DNA sequences present in the genome of Brassica nigra has been characterized by cloning and sequencing of the repeat element. It is a novel tandem repeat, different from any other known plant repetitive DNA sequences. The monomeric unit is 348 bp in length and highly AT-rich (74%) as compared to other reported plant tandem repeated DNA sequences. The average copy number of the cloned repeat is 1 × 105 per haploid genome and constitutes approximately 2.5% of the total B. nigra genome. It shows no sequence homology with the 177 bp satellite DNA sequences of other crucifers as well as with other plant tandem repeated DNA. The distribution and evolution of this tandem repeat in crucifers is discussed. © 1990.

引用

页码：223 / 229

页数：7

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