CHEMILUMINESCENCE FROM THE REACTION OF CHLOROAURIC ACID WITH LUMINOL IN REVERSE MICELLES

The chemiluminescence (CL) reaction of tetrachloroaurate(III) with luminol in reversed micellar system of hexadecyltrimethylammonium chloride-water (buffered with sodium carbonate)-6:5 (v/v) chloroform-cyclohexane was studied and applied to develop a new flow injection procedure for the determination of gold at trace levels in chloroform. The behavior of the gold(III) complex was examined by its spectral measurements in the near-UV and visible regions in different media. All the optimization conditions for the method were determined by using a flow system, and the characteristics of the CL system in the reverse micelles were examined. The method is simple and reproducible with a detection limit (DL) of 10 pg mL-1. The calibration graph was linear between 1 ng mL-1 and 1000 ng mL-1 along with a second straight line from the DL up to 1 ng mL-1. The relative standard deviation was 3%.