THE ACRIDINE-ORANGE TEST - DETERMINING THE RELATIONSHIP BETWEEN SPERM MORPHOLOGY AND FERTILIZATION INVITRO

被引：48

作者：

CLAASSENS, OE ^{[1
]}

MENKVELD, R ^{[1
]}

FRANKEN, DR ^{[1
]}

PRETORIUS, E ^{[1
]}

SWART, Y ^{[1
]}

LOMBARD, CJ ^{[1
]}

KRUGER, TF ^{[1
]}

机构：

[1] TYGERBERG HOSP,DEPT OBSTET & GYNAECOL,REPROD BIOL UNIT,PAROWVALLEI,SOUTH AFRICA

来源：

HUMAN REPRODUCTION | 1992年 / 7卷 / 02期

关键词：

ACRIDINE ORANGE TEST; SPERM DNA STATUS; MORPHOLOGY; FERTILIZATION INVITRO;

D O I：

10.1093/oxfordjournals.humrep.a137625

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

The prediction of human fertilization is an important aspect of research protocols dealing with male fertility. Sperm DNA has been reported to be an indicator of human sperm fertility potential. The Acridine Orange test for evaluation of sperm DNA has been employed during the present study to determine its relationship with human sperm morphology and fertilization in vitro. Seventy-six patients from the in-vitro fertilization (IVF) and/or gamete intra-Fallopian transfer (GIFT) programme were randomly selected for the study. All patients had a routine semen analysis, sperm DNA evaluation and underwent standard IVF procedures at the time of the study. The results indicated a moderate positive correlation (r = 0.38, P = 0.0006) between results of the Acridine Orange test and normal sperm morphology. Patients with an Acridine Orange test value exceeding 24% had significantly higher oocyte fertilization rates than patients with lower values, for metaphase I (74 versus 51%, P = 0.0008) and for metaphase II oocytes (88 versus 60%, P = 0.0001). Sperm morphology, however, proved to be a more significant predictor of fertilization in vitro compared to the Acridine Orange test.

引用

页码：242 / 247

页数：6

共 21 条

[1] Burkman L.J., Coddington C.C., Franken D.R., Kruger T.F., Rosenwaks Z., Hodgen G.D., The hemizona assay (HZA): Development of a diagnostic test for the binding of human spermatozoa to the human hemizona pellucida to predict fertilization potential, Fertil. Steril, 46, pp. 688-697, (1988)
[2] Chan S.Y.W., Fox E.J., Chan M.M.C., Tsoi W., Wang C., Tang L.C.H., Tang G.W.K., Ho P., The relationship between the human sperm hypoosmotic swelling test, routine semen analysis, and the human sperm zona-free hamster ovum penetration assay, Fertil. Steril, 44, pp. 668-672, (1985)
[3] Darzynkiewicz Z., Traganos F., Sharpless T., Melamed M.R., Thermal denaturation of DNA in situ as studied by Acridine Orange test staining and automated cytofluorometry, Exp. Cell Res, 90, pp. 411-428, (1975)
[4] Evenson D.P., Darzynkiewicz Z., Melamed M.R., Relation of mammalian sperm chromatin heterogeneity to fertility, Science, 21, pp. 1131-1133, (1980)
[5] Fleiss J.L., Statistical Methods for Rates and Propoaions, (1981)
[6] Hinting A., Comhaire F., Vermeulen L., Dhont M., Vermeulen A., Vandekerchkhove D., Value of sperm characteristics and the result of in-vitro fertilization for predicting the outcome of assisted reproduction, Int. J. Androl, 13, pp. 59-66, (1990)
[7] Ibrahim M.E., Moussa M.A., Pedersen H., Sperm chromatin heterogeneity as an infertility factor, Arch. Androl, 21, pp. 129-133, (1988)
[8] Kruger T.F., Menkveld R., Stander F.S.H., Lombard C.J., Der Merwe J.P., Zyl J.A., Smith K., Sperm morphologic features as a prognostic factor in in vitro fertilization, Fertil. Steril, 46, pp. 1118-1123, (1986)
[9] Kruger T.F., Acosta A.A., Simmons K.F., Swanson J.R., Matta J.F., Oehninger S., Predictive value of abnormal sperm morphology in in vitro fertilization, Fertil. Steril, 49, pp. 112-117, (1988)
[10] Liu D.Y., Baker G.H.W., The proportion of human sperm with poor morphology but normal intact acrosomes detected with Pisum sativum agglutinin correlates with fertilization in vitro, Fertil. Steril, 50, pp. 288-293, (1988)

← 1 2 3 →