ROD-LIKE CHOLESTEROL MICELLES IN AQUEOUS-SOLUTION STUDIED USING POLARIZED AND DEPOLARIZED DYNAMIC LIGHT-SCATTERING

被引:36
作者
CASTANHO, MARB
BROWN, W
PRIETO, MJE
机构
[1] UNIV UPPSALA,INST PHYS CHEM,BOX 532,S-75121 UPPSALA,SWEDEN
[2] CTR QUIM FIS MOLEC,P-1096 LISBON,PORTUGAL
关键词
D O I
10.1016/S0006-3495(92)81733-2
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Micelles of cholesterol in aqueous solution have been investigated using polarized and depolarized dynamic light scattering. They are shown to be highly extended and characterized by a narrow size distribution. It is shown that a rod-like model is applicable with length, L = 580 nm. Determination of the rotational diffusion coefficient by analysis of the autocorrelation function gave a value of THETA = 150 s-1, which is close to the calculated value for the rod with this dimension. Depolarized dynamic light scattering measurements as a function of angle gave a value of 110 s-1.
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收藏
页码:1455 / 1461
页数:7
相关论文
共 37 条
[1]   PHYSICOCHEMICAL BASIS OF CHOLESTEROL GALLSTONE FORMATION IN MAN [J].
ADMIRAND, WH ;
SMALL, DM .
JOURNAL OF CLINICAL INVESTIGATION, 1968, 47 (05) :1043-&
[2]  
BERNE BJ, 1976, DYNAMIC LIGHT SCATTE, P177
[4]   ROTATIONAL DIFFUSION CONSTANT OF A CYLINDRICAL PARTICLE [J].
BROERSMA, S .
JOURNAL OF CHEMICAL PHYSICS, 1960, 32 (06) :1626-1631
[5]   VISCOUS FORCE CONSTANT FOR A CLOSED CYLINDER [J].
BROERSMA, S .
JOURNAL OF CHEMICAL PHYSICS, 1960, 32 (06) :1632-1635
[6]   THE SOLUBILITY OF CHOLESTEROL AND ITS EXCHANGE BETWEEN MEMBRANES [J].
BRUCKDORFER, KR ;
SHERRY, MK .
BIOCHIMICA ET BIOPHYSICA ACTA, 1984, 769 (01) :187-196
[7]  
Burchard W., 1992, LASER LIGHT SCATTERI, P3
[8]  
CASTANHO MARB, 1992, J BIOL CHEM, V267, P204
[9]   CRYSTAL-STRUCTURE OF CHOLESTEROL MONOHYDRATE [J].
CRAVEN, BM .
NATURE, 1976, 260 (5553) :727-729
[10]  
Doi M., 1986, THEORY POLYM DYNAMIC, P289