HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF HUMAN GLYCOPROTEIN HORMONES

被引：11

作者：

CHLENOV, MA

KANDYBA, EI

NAGORNAYA, LV

ORLOVA, IL

VOLGIN, YV

机构：

[1] NPO Biotechnologia, 117246 Moscow

来源：

JOURNAL OF CHROMATOGRAPHY | 1993年 / 631卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(93)80531-C

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The chromatographic behaviour of the glycoprotein hormones from human pituitary glands and of placental origin [thyroid-stimulating hormone, luteinizing hormone and chorionic gonadotropin (CG)] was studied. It was shown that hydrophobic interaction chromatography on a microparticulate packing and anion-exchange HPLC can be applied for the purification of these hormones. Reversed-phase HPLC on wide-pore C4-bonded silica at neutral pH can be applied for the determination of the above hormones and for the isolation of pure CG and its subunits.

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页码：261 / 267

页数：7

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