SYNTHESIS OF TRINITROPHENYLAMINOLAURIC ACID AND USE OF ITS GLYCERYL ESTERS FOR ASSAYING LIPASE BY A SPECTROPHOTOMETRIC PROCEDURE

被引：16

作者：

GOLDBERG, R ^{[1
]}

BARENHOLZ, Y ^{[1
]}

GATT, S ^{[1
]}

机构：

[1] HEBREW UNIV JERUSALEM,HADASSAH MED SCH,DEPT BIOCHEM,NEUROCHEM LAB,JERUSALEM 91000,ISRAEL

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1978年 / 531卷 / 02期

关键词：

D O I：

10.1016/0005-2760(78)90148-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Trinitrophenylaminolauric acid was synthesized from ω-aminolauric acid and trinitrobenzenesulfonic acid and then condensed with glycerol to yield mono-, di- and triacylglyceryl esters of this acid. Hydrolysis of these glycerides was followed by isolation of the yellow fatty acid with the aid of one solvent extraction step and estimating its content by spectrophotometry. This procedure was used to assay the activities of lipases from hog pancreas, rat bile, microsomes of rat brain and Rhizopus arrhizus delamar. © 1978.

引用

页码：237 / 241

页数：5

共 24 条

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