CLOSTRIDIUM PERFRINGENS POLYNUCLEOTIDE PHOSPHORYLASE - INTERCONVERSION OF HEAVY AND LIGHT MOLECULAR WEIGHT SPECIES

被引：4

作者：

GUISSANI, A

GRUNBERG.M

机构：

[1] Institut de Biologie Physico-chimique, Paris, rue P. Curie

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1969年 / 35卷 / 01期

关键词：

D O I：

10.1016/0006-291X(69)90494-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Evidence is presented showing that the 200 000 species (S20, W = 9S) of Clostridium perfringens polynucleotide phosphorylase is partially converted into a 70 000 species (S20, W = 4.5S) by 30 minutes preincubation at 25°C with β-mercaptoethanol. The two species, which have different catalytic properties, can also be distinguished by their different mobility through disc electrophoresis technique on acrylamide gel. Interconversion of the two species is studied. © 1969.

引用

页码：131 / &

共 7 条

[1] CLOSTRIDIUM PERFRINGENS POLYNUCLEOTIDE PHOSPHORYLASE - 2 MOLECULAR SPECIES [J].