NEW ELECTROPHORETIC METHOD FOR THE COMPLETE SEPARATION OF ALL KNOWN ANIMAL GLYCOSAMINOGLYCANS IN A MONODIMENSIONAL RUN

A new rapid, sensitive, and reproducible method is presented which allows the separation of all known animal glycosaminoglycans (including two subclasses of heparan sulfate, chondroitin 4-sulfate, chondroitin 6-sulfate, dermatan sulfate, hyaluronic acid, heparin, and keratan sulfate) in a single monodimensional electrophoresis on cellulose acetate. A very narrow starting band is obtained by formation of a discontinuity in the electric field and all eight compounds are then selectively resolved by combining their migration properties in a barium acetate buffer and their differential sensitivity to precipitation by ethanol. © 1979 Academic Press, Inc.