A SIMPLE METHOD FOR SITE-DIRECTED MUTAGENESIS USING THE POLYMERASE CHAIN-REACTION

被引：542

作者：

HEMSLEY, A

ARNHEIM, N

TONEY, MD

CORTOPASSI, G

GALAS, DJ

机构：

[1] UNIV SO CALIF,DEPT MOLEC BIOL,LOS ANGELES,CA 90089

[2] UNIV CALIF BERKELEY,DEPT BIOCHEM,BERKELEY,CA 94720

来源：

NUCLEIC ACIDS RESEARCH | 1989年 / 17卷 / 16期

关键词：

D O I：

10.1093/nar/17.16.6545

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

引用

收藏

页码：6545 / 6551

页数：7

相关论文

共 16 条

[1] BIRNBOIM HC, 1979, NUCLEIC ACIDS RES, V7, P1513
[2] NOVEL NON-TEMPLATED NUCLEOTIDE ADDITION-REACTIONS CATALYZED BY PROCARYOTIC AND EUKARYOTIC DNA-POLYMERASES
CLARK, JM
[J]. NUCLEIC ACIDS RESEARCH, 1988, 16 (20) : 9677 - 9686
[3] A SIMPLE AND EFFICIENT PROCEDURE FOR SATURATION MUTAGENESIS USING MIXED OLIGODEOXYNUCLEOTIDES
DERBYSHIRE, KM
SALVO, JJ
GRINDLEY, NDF
[J]. GENE, 1986, 46 (2-3) : 145 - 152
[4] A GENERAL-METHOD OF INVITRO PREPARATION AND SPECIFIC MUTAGENESIS OF DNA FRAGMENTS - STUDY OF PROTEIN AND DNA INTERACTIONS
HIGUCHI, R
KRUMMEL, B
SAIKI, RK
[J]. NUCLEIC ACIDS RESEARCH, 1988, 16 (15) : 7351 - 7367
[5] AMPLIFICATION OF HUMAN MINISATELLITES BY THE POLYMERASE CHAIN-REACTION - TOWARDS DNA FINGERPRINTING OF SINGLE CELLS
JEFFREYS, AJ
WILSON, V
NEUMANN, R
KEYTE, J
[J]. NUCLEIC ACIDS RESEARCH, 1988, 16 (23) : 10953 - 10971
[6] USE OF POLYMERASE CHAIN-REACTION CATALYZED BY TAQ-DNA POLYMERASE FOR SITE-SPECIFIC MUTAGENESIS
KADOWAKI, H
KADOWAKI, T
WONDISFORD, FE
TAYLOR, SI
[J]. GENE, 1989, 76 (01) : 161 - 166
[7] THE GAPPED DUPLEX DNA APPROACH TO OLIGONUCLEOTIDE-DIRECTED MUTATION CONSTRUCTION
KRAMER, W
DRUTSA, V
JANSEN, HW
KRAMER, B
PFLUGFELDER, M
FRITZ, HJ
[J]. NUCLEIC ACIDS RESEARCH, 1984, 12 (24) : 9441 - 9456
[8] Maniatis T., 1982, MOL CLONING
[9] MULLIS KB, 1987, METHOD ENZYMOL, V155, P335
[10] OCHMAN H, 1988, GENETICS, V120, P621