THE EFFECTS OF SAMPLE PREPARATION, PYROLYSIS AND PYROLYZATE TRANSFER CONDITIONS ON PYROLYSIS MASS-SPECTRA

被引：41

作者：

WINDIG, W ^{[1
]}

KISTEMAKER, PG ^{[1
]}

HAVERKAMP, J ^{[1
]}

机构：

[1] UNIV UTAH,FLAMMABIL RES CTR,SALT LAKE CITY,UT 84108

来源：

JOURNAL OF ANALYTICAL AND APPLIED PYROLYSIS | 1979年 / 1卷 / 01期

关键词：

D O I：

10.1016/0165-2370(79)80004-2

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Two different biopolymers, bovine serum albumin and glycogen, were examined by pyrolysis-mass spectrometry under various experimental conditions. The parameters tested include the method of cleaning the pyrolysis wire, the choice of the sample solvent, the sample size, the temperature-time profile and the temperature of the mass spectrometric inlet system. In order to determine quantitatively the effects of changes in experimental conditions on reproducibility of the spectra, the differences observed between the pyrolysis mass spectra represented as numerical dissimilarity values. It is concluded that the long-term differences observed are only partly explained by fluctuations in the conditions studied. However, it appears feasible to define a set of standard conditions with respect to the production of the pyrolyzate which should afford a reasonable degree of interlaboratory reproducibility. © 1979.

引用

页码：39 / 52

页数：14

共 31 条

[1] IDENTIFICATION OF BACTERIA USING MASS-SPECTROMETRY [J].