SEPARATION OF NITROGENASE SYSTEM OF AZOTOBACTER INTO 3 COMPONENTS AND PURIFICATION OF ONE OF COMPONENTS

被引：12

作者：

KAJIYAMA, S

MATSUKI, T

NOSOH, Y

机构：

[1] The Laboratory of Chemistry of Natural Products, Tokyo Institute of Technology, Meguroku, Tokyo

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1969年 / 37卷 / 04期

关键词：

D O I：

10.1016/0006-291X(69)90869-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The nitrogenase system of Azotobacter vinelandii is separated into three components by column chromatographies on Sephadex G-50 and DEAE-cellulose. The components, when all of them are present together, exhibit the nitrogenase activity. One of the components is purified. to a state, homogeneous ultracentrifugally and. electrophoretically. The sedimentation coefficient and molecular weight are respectively 5.8 S and 93,000. The component has an absorption maximum at 416 mμ, which is shifted to 418 mμ upon reduction with dithionite, and contains Fe and Zn atoms. © 1969.

引用

页码：711 / &

共 7 条

[1] NITROGENASE SYSTEM FROM AZOTOBACTER - 2-ENZYME REQUIREMENT FOR N2 REDUCTION ATP-DEPENDENT H2 EVOLUTION AND ATP HYDROLYSIS [J].