A system is described for assembling infectious bacteriophage ∅6 nucleocapsids in vitro. Procapsids encoded by cDNA copies of genomic segment L in Escherichia coli were used to package and replicate viral RNA segments. The resulting filled particles were shown to be capable of infecting host cell spheroplasts after incubation with purified nucleocapsid shell protein P8. The infected spheroplasts yielded infectious virions. A modified cDNA-derived RNA segment was inserted into virions by this method. The resulting infectious virions contained the same 4-base-pair deletion as the modified cDNA. These findings support the contention that the preformed procapsids are the 'machine' that replicates the ∅6 genome, by showing that the cDNA-derived procapsids are competent to package and replicate RNA properly.
机构:
CUNY MT SINAI SCH MED, DEPT MICROBIOL, 1 GUSTAVE L LEVY PL, NEW YORK, NY 10029 USACUNY MT SINAI SCH MED, DEPT MICROBIOL, 1 GUSTAVE L LEVY PL, NEW YORK, NY 10029 USA
ENAMI, M
;
LUYTJES, W
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CUNY MT SINAI SCH MED, DEPT MICROBIOL, 1 GUSTAVE L LEVY PL, NEW YORK, NY 10029 USACUNY MT SINAI SCH MED, DEPT MICROBIOL, 1 GUSTAVE L LEVY PL, NEW YORK, NY 10029 USA
LUYTJES, W
;
KRYSTAL, M
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CUNY MT SINAI SCH MED, DEPT MICROBIOL, 1 GUSTAVE L LEVY PL, NEW YORK, NY 10029 USACUNY MT SINAI SCH MED, DEPT MICROBIOL, 1 GUSTAVE L LEVY PL, NEW YORK, NY 10029 USA
KRYSTAL, M
;
PALESE, P
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机构:
CUNY MT SINAI SCH MED, DEPT MICROBIOL, 1 GUSTAVE L LEVY PL, NEW YORK, NY 10029 USACUNY MT SINAI SCH MED, DEPT MICROBIOL, 1 GUSTAVE L LEVY PL, NEW YORK, NY 10029 USA
机构:
CUNY MT SINAI SCH MED, DEPT MICROBIOL, 1 GUSTAVE L LEVY PL, NEW YORK, NY 10029 USACUNY MT SINAI SCH MED, DEPT MICROBIOL, 1 GUSTAVE L LEVY PL, NEW YORK, NY 10029 USA
ENAMI, M
;
LUYTJES, W
论文数: 0引用数: 0
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机构:
CUNY MT SINAI SCH MED, DEPT MICROBIOL, 1 GUSTAVE L LEVY PL, NEW YORK, NY 10029 USACUNY MT SINAI SCH MED, DEPT MICROBIOL, 1 GUSTAVE L LEVY PL, NEW YORK, NY 10029 USA
LUYTJES, W
;
KRYSTAL, M
论文数: 0引用数: 0
h-index: 0
机构:
CUNY MT SINAI SCH MED, DEPT MICROBIOL, 1 GUSTAVE L LEVY PL, NEW YORK, NY 10029 USACUNY MT SINAI SCH MED, DEPT MICROBIOL, 1 GUSTAVE L LEVY PL, NEW YORK, NY 10029 USA
KRYSTAL, M
;
PALESE, P
论文数: 0引用数: 0
h-index: 0
机构:
CUNY MT SINAI SCH MED, DEPT MICROBIOL, 1 GUSTAVE L LEVY PL, NEW YORK, NY 10029 USACUNY MT SINAI SCH MED, DEPT MICROBIOL, 1 GUSTAVE L LEVY PL, NEW YORK, NY 10029 USA