THE BRAIN RYANODINE RECEPTOR - A CAFFEINE-SENSITIVE CALCIUM RELEASE CHANNEL

被引:355
作者
MCPHERSON, PS
KIM, YK
VALDIVIA, H
KNUDSON, CM
TAKEKURA, H
FRANZINIARMSTRONG, C
CORONADO, R
CAMPBELL, KP
机构
[1] UNIV WISCONSIN,SCH MED,DEPT PHYSIOL,MADISON,WI 53706
[2] UNIV PENN,DEPT BIOL & ANAT,PHILADELPHIA,PA 19104
关键词
D O I
10.1016/0896-6273(91)90070-G
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The release of stored Ca2+ from intracellular pools triggers a variety of important neuronal processes. Physiological and pharmacological evidence has indicated the presence of caffeine-sensitive intracellular pools that are distinct from the well-characterized inositol 1,4,5,-trisphosphate (IP3)-gated pools. Here we report that the brain ryanodine receptor functions as a caffeine- and ryanodine-sensitive Ca2+ release channel that is distinct from the brain IP3 receptor. The brain ryanodine receptor has been purified 6700-fold with no change in [H-3]ryanodine binding affinity and shown to be a homotetramer composed of an approximately 500 kd protein subunit, which is identified by anti-peptide antibodies against the skeletal and cardiac muscle ryanodine receptors. Our results demonstrate that the brain ryanodine receptor functions as a caffeine-sensitive Ca2+ release channel and thus is the likely gating mechanism for intracellular caffeine-sensitive Ca2+ pools in neurons.
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页码:17 / 25
页数:9
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