FERTILE PLANT-REGENERATION FROM PROTOPLASTS OF MEADOW FESCUE (FESTUCA-PRATENSIS HUDS)

被引：37

作者：

WANG, ZY ^{[1
]}

VALLES, MP ^{[1
]}

MONTAVON, P ^{[1
]}

POTRYKUS, I ^{[1
]}

SPANGENBERG, G ^{[1
]}

机构：

[1] SWISS FED INST TECHNOL,INST PLANT SCI,CH-8092 ZURICH,SWITZERLAND

来源：

PLANT CELL REPORTS | 1993年 / 12卷 / 02期

关键词：

FORAGE GRASSES; FESTUCA-PRATENSIS; SUSPENSION CULTURES; PROTOPLASTS; PLANT REGENERATION;

D O I：

10.1007/BF00241942

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Suspension cultures from mature embryo-derived compact callus were initiated in seven meadow fescue (Festuca pratensis Huds.) cultivars. Four to six months after initiation, embryogenic suspension cultures with a moderate growth rate were established from three of them (cvs. Barmondo, Belimo and Leopard). These suspension cultures showed the capacity, maintained over six months, to regenerate green plants which could be grown to maturity under greenhouse conditions. Morphogenic suspension cultures from single genotypes of three F. pratensis cultivars (cvs. Barmondo, Belimo and Leopard) yielded large numbers of protoplasts, which upon culture in agarose beads using nurse cells formed microcalli with an overall plating efficiency in the range of 10(-3) to 10(-4). Mature plants were reproducibly regenerated and established in soil, from such protoplasts during a period of six months. The regeneration of fertile plants from protoplasts derived from suspension cultures of meadow fescue and its implications on gene transfer technology for this species are discussed.

引用

页码：95 / 100

页数：6

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