SP1 CAN DISPLACE GHF-1 FROM ITS DISTAL BINDING-SITE AND STIMULATE TRANSCRIPTION FROM THE GROWTH-HORMONE GENE PROMOTER

被引：48

作者：

LEMAIGRE, FP

LAFONTAINE, DA

COURTOIS, SJ

DURVIAUX, SM

ROUSSEAU, GG

机构：

[1] CATHOLIC UNIV LOUVAIN,SCH MED,HORMONE & METAB RES UNIT,75 AVE HIPPOCRATE,B-1200 BRUSSELS,BELGIUM

[2] INT INST CELLULAR & MOLEC PATHOL,B-1200 BRUSSELS,BELGIUM

来源：

MOLECULAR AND CELLULAR BIOLOGY | 1990年 / 10卷 / 04期

关键词：

D O I：

10.1128/MCB.10.4.1811

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

DNase I footprinting experiments showed that binding activities of Sp1 and of GHF-1 to its distal site on the human growth hormone gene promoter are mutually exclusive. The kinetics of GHF-1 binding were indicative of positive cooperativity. The Sp1 site did not affect promoter activity in cell-free transcription. Still, Sp1 could compensate partially for the decreased stimulation of transcription seen at low GHF-1 concentrations.

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页码：1811 / 1814

页数：4

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