7,4'-DIHYDROXYFLAVANONE IS THE MAJOR AZORHIZOBIUM-NOD GENE-INDUCING FACTOR PRESENT IN SESBANIA-ROSTRATA SEEDLING EXUDATE

被引：15

作者：

MESSENS, E

GEELEN, D

VANMONTAGU, M

HOLSTERS, M

机构：

[1] Laboratorium voor Genetica, Rijksuniversiteit Gent, B-9000 Gent

来源：

MOLECULAR PLANT-MICROBE INTERACTIONS | 1991年 / 4卷 / 03期

关键词：

GAS CHROMATOGRAPHY; LEGUMINOUS PLANTS; MASS SPECTROMETRY; SYMBIOSIS;

D O I：

10.1094/MPMI-4-262

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Exudate from Sesbania rostrata seedlings contains signaling compounds that induce the common nodABC operon of the bacterial symbiont Azorhizobium caulinodans ORS571. An Azorhizobium strain harboring a nodA::lacZ reporter fusion was used to monitor the nod-inducing activity of crude exudate fractions that were separated by reversed-phase chromatography. The major inducer was shown, by spectroscopic analysis and by comparison with chemically synthesized compounds, to be 7,4'-dihydroxyflavanone (liquiritigenin). Newly synthesized analogues, 7,3'-dihydroxyflavanone and 7,2'-dihydroxyflavanone, have only poor inducing activity.

引用

页码：262 / 267

页数：6

共 25 条

[1]

Achenbach H., Stocker M., Constenla M.A., Flavonoid other constituents of Bauhinia manca, Phytochemistry, 27, pp. 1835-1845, (1988)

[2]

Dreyfus B., Dommergues Y.R., Nitrogen-fixing nodules induced by Rhizobium on the stem of the tropical legume Sesbania rostrata, FEMS Microbiol. Lett, 10, pp. 313-317, (1981)

[3]

Dreyfus B., Garcia J.L., Gillis M., Characterization of Azorhizobium caulinodans gen. nov., sp. nov., a stem-nodulating nitrogen-fixing bacterium isolated from Sesbania rostrata, International Journal of Systematic Bacteriology, 38, 1, pp. 89-98, (1988)

[4]

Firmin J.L., Wilson K.E., Rossen I., Johnston A.W.B., Flavonoid activation of nodulation genes in Rhizobium reversed by other compounds present in plants, Nature, 324, 6092, pp. 90-92, (1986)

[5]

Fisher R.F., Egelhoff T.T., Mulligan J.T., Long S.R., Specific binding of proteins from Rhizobium meliloti cell-free extracts containing NodD to DNA sequences upstream of inducible nodulation genes, Genes Dev, 2, pp. 282-293, (1988)

[6]

Goethals K., Gao M., Tomekpe K., Van Montagu M., Holsters M., Common nod ABC genes in Nod locus 1 of Azorhizobium caulinodans: Nucleotide sequence and plant-inducible expression, Mol. Gen. Genet, 219, pp. 289-298, (1989)

[7]

Goethals K., Van Den Eede G., Van Montagu M., Holsters M., Identification and characterization of a functional nodD gene in Azorhizobium caulinodans ORS571, Journal of Bacteriology, 172, 5, pp. 2658-2666, (1990)

[8]

Hong G.-F., Burn J.E., Johnston B A.W., Evidence that DNA involved in the expression of nodulation (nod) genes in Rhizobium binds to the product of the regulatory gene nodD, Nucleic Acids Res, 15, pp. 9677-9690, (1987)

[9]

Horvath B., Bachem C.W.B., Schell J., Kondorosi A., Host-specific regulation of nodulation genes in Rhizobium is mediated by a plant-signal, interacting with the nodD gene product, EMBO J., 6, pp. 841-848, (1987)

[10]

Ingham J.L., Isoflavonoid phytoalexins of the genus Medicago, Biochem. Syst. Ecol, 7, pp. 29-34, (1979)

← 1 2 3 →