NUCLEOTIDE-SEQUENCE AND STRUCTURAL RELATIONSHIPS OF A CHLORAMPHENICOL ACETYLTRANSFERASE ENCODED BY THE PLASMID PSCS6 FROM STAPHYLOCOCCUS-AUREUS

被引：13

作者：

CARDOSO, M ^{[1
]}

SCHWARZ, S ^{[1
]}

机构：

[1] UNIV GIESSEN,INST BAKTERIOL & IMMUNOL,FRANKFURTER STR 107,W-6300 GIESSEN,GERMANY

来源：

JOURNAL OF APPLIED BACTERIOLOGY | 1992年 / 72卷 / 04期

关键词：

D O I：

10.1111/j.1365-2672.1992.tb01837.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The 4.6 kb chloramphenicol resistance (Cm(R)) plasmid, pSCS6, isolated from a naturally occurring Staphylococcus aureus biotype C encoded an inducible chloramphenicol acetyltransferase (CAT). The respective cat gene and its regulatory region were cloned. Sequence analyses revealed two open reading frames: one for a 9-amino acid leader peptide and the other for the 215-amino acid CAT monomer. Comparisons of the predicted CAT amino acid sequences revealed a high degree of similarity between CAT from pSCS6 and the CAT variants encoded by Cm(R) plasmids of the pC221 family. These close structural relationships suggested an intraspecific exchange of Cm(R)-determinants between Staph. aureus of human and bovine biotype.

引用

页码：289 / 293

页数：5

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