THE COMPLETE PRIMARY STRUCTURE OF ABRIN-A B-CHAIN

被引：19

作者：

CHEN, YL

CHOW, LP

TSUGITA, A

LIN, JY

机构：

[1] NATL TAIWAN UNIV, COLL MED, INST BIOCHEM, TAIPEI 10018, TAIWAN

[2] UNIV TOKYO, BIOSCI RES INST, YAMAZAKI, YAMAZAKI 278, JAPAN

来源：

FEBS LETTERS | 1992年 / 309卷 / 02期

关键词：

ABRIN-A B-CHAIN; PRIMARY STRUCTURE;

D O I：

10.1016/0014-5793(92)81076-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The complete 267 amino-acid sequence of abrin-a B chain was determined by analysis of peptides obtained by digestion with trypsin, chymotrypsin, lysyl endopeptidase, Staphylococcus aureus V8 protease and thermolysin. The sequence is not identical with that predicted previously by nucleotide sequencing, indicating the presence of isoforms of abrin. Comparison of the amino acid sequence of abrin-a B chain with that of ricin-D B chain reveals a high degree of sequence identity (59%). Abrin-a B chain appears to consist of two domains, each domain with subdomains (alpha, beta, gamma) of about 40 amino acid residues.

引用

页码：115 / 118

页数：4

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