INSTABILITY OF RICKETTSIA-PROWAZEKII RNA POLYMERASE-PROMOTER COMPLEXES

被引：5

作者：

ANISKOVITCH, LP ^{[1
]}

WINKLER, HH ^{[1
]}

机构：

[1] UNIV SO ALABAMA,COLL MED,DEPT MICROBIOL & IMMUNOL,MOLEC BIOL LAB,MOBILE,AL 36688

来源：

JOURNAL OF BACTERIOLOGY | 1995年 / 177卷 / 21期

关键词：

D O I：

10.1128/jb.177.21.6301-6303.1995

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The Rickettsia prowazekii sigma factor was overexpressed, purified, and used to reconstitute RNA polymerase holoenzyme species. R. prowazekii RNA polymerase-promoter complexes were unstable and remained dissociable and heparin sensitive under conditions in which the corresponding Escherichia call complexes were not. The R. prowazekii core played the major role in determining heparin sensitivity.

引用

页码：6301 / 6303

页数：3

共 12 条

[1] Ausubel F. M., 1989, CURRENT PROTOCOLS MO, V1
[2] AUSUBEL FM, 1989, CURRENT PROTOCOLS MO, V2
[3] BORUKHOV S, 1993, J BIOL CHEM, V268, P23477
[4] DING HF, 1993, J BIOL CHEM, V268, P3897
[5] PURIFICATION AND PARTIAL CHARACTERIZATION OF THE DNA-DEPENDENT RNA-POLYMERASE FROM RICKETTSIA-PROWAZEKII
DING, HF
WINKLER, HH
[J]. JOURNAL OF BACTERIOLOGY, 1990, 172 (10) : 5624 - 5630
[6] THE MOLAR RATIO OF SIGMA(73) TO CORE POLYMERASE IN THE OBLIGATE INTRACELLULAR BACTERIUM, RICKETTSIA-PROWAZEKII
DING, HF
WINKLER, HH
[J]. MOLECULAR MICROBIOLOGY, 1994, 11 (05) : 869 - 873
[7] VISUALIZATION AND QUANTITATIVE-ANALYSIS OF COMPLEX-FORMATION BETWEEN ESCHERICHIA-COLI RNA-POLYMERASE AND AN RIBOSOMAL-RNA PROMOTER INVITRO
GOURSE, RL
[J]. NUCLEIC ACIDS RESEARCH, 1988, 16 (20) : 9789 - 9809
[8] LANGERT W, 1991, J BIOL CHEM, V266, P21608
[9] FACTOR-INDEPENDENT ACTIVATION OF ESCHERICHIA-COLI RIBOSOMAL-RNA TRANSCRIPTION .1. KINETIC-ANALYSIS OF THE ROLES OF THE UPSTREAM ACTIVATOR REGION AND SUPERCOILING ON TRANSCRIPTION OF THE RRNB P1 PROMOTER INVITRO
LEIRMO, S
GOURSE, RL
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1991, 220 (03) : 555 - 568
[10] ISOLATION AND CHARACTERIZATION OF THE GENE CODING FOR THE MAJOR SIGMA-FACTOR OF RICKETTSIA-PROWAZEKII DNA-DEPENDENT RNA-POLYMERASE
MARKS, GL
WINKLER, HH
WOOD, DO
[J]. GENE, 1992, 121 (01) : 155 - 160

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