ISOLATION AND STRUCTURAL-ANALYSIS OF 3 NEW DISIALYLATED OLIGOSACCHARIDES FROM HUMAN-MILK

Five disialylated oligosaccharides, of which three were new compounds, were isolated from pooled human milk. The isolation procedure included gel filtration and ion-exchange chromatography and the final purification was accomplished by HPLC using a mobile phase containing triethylamine as an ion-pairing reagent. Structural analysis was carried out by chemical analyses, fast atom bombardment mass spectrometry, and 500-MHz NMR spectroscopy. The following new structures were found: {A figure is presented}. © 1990.