FACILE DETERMINATION OF THE SPECIFIC ACTIVITY OF CARBONYL-COMPOUNDS REDUCED BY TRITIATED BOROHYDRIDE

被引:2
作者
AVIGAD, G
机构
[1] Department of Biochemistry, College of Medicine and Dentistry, New Jersey-Rutgers Medical School, Piscataway
关键词
D O I
10.1016/S0003-2670(01)93279-9
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Three procedures are described for microliter samples of glucose 6-phosphate or lactose as model compounds. After the reduction with [3H]-NaBH4 and suitable treatment, specific activity is calculated from the ratios 3H activity/total phosphorus, 3H/14C activity, or 3H activity/galactoside concentration. © 1979.
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页码:315 / 319
页数:5
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