CLONING AND CHARACTERIZATION OF 2 GENES FROM STREPTOMYCES-LIVIDANS THAT CONFER INDUCIBLE RESISTANCE TO LINCOMYCIN AND MACROLIDE ANTIBIOTICS

被引：72

作者：

JENKINS, G ^{[1
]}

CUNDLIFFE, E ^{[1
]}

机构：

[1] UNIV LEICESTER,DEPT BIOCHEM,LEICESTER LE1 7RH,ENGLAND

来源：

GENE | 1991年 / 108卷 / 01期

基金：

英国医学研究理事会;

关键词：

RECOMBINANT DNA; RIBOSOMAL RNA METHYLTRANSFERASE; GLYCOSYL TRANSFERASE; LRM; MGT GENES;

D O I：

10.1016/0378-1119(91)90487-V

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Inducible resistance to lincomycin and macrolides in Streptomyces lividans TK21 results from expression of two linked genes: lrm, encoding a ribosomal RNA methyltransferase that confers high-level resistance to lincomycin with lower levels of resistance to macrolides, and mgt, encoding a glycosyl transferase that specifically inactivates macrolides using UDP-glucose as cofactor. The lrm and mgt genes have been cloned and sequenced. The deduced lrm product is a 26-kDa protein with much similarity to other ribosomal RNA methyltransferases, such as the carB, tlrA and ermE products, whereas the mgt product (predicted to be 42 kDa) resembles a eukaryotic glycosyl transferase. Macrolides that induce the lrm-mgt gene pair are substrates for inactivation by the mgt product, and the lrm product confers ribosomal resistance to such inducers.

引用

页码：55 / 62

页数：8

共 24 条

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