FUNCTIONAL DIFFERENCE BETWEEN SERCA2A AND SERCA2B CA2+ PUMPS AND THEIR MODULATION BY PHOSPHOLAMBAN

被引：125

作者：

VERBOOMEN, H

WUYTACK, F

DESMEDT, H

HIMPENS, B

CASTEELS, R

机构：

[1] Laboratorium voor Fysiologie, Katholieke Universiteit Leuven, Campus Gasthuisberg, B-3000 Leuven

来源：

BIOCHEMICAL JOURNAL | 1992年 / 286卷

关键词：

D O I：

10.1042/bj2860591

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

COS 1 cells were transfected with full-length pig stomach sarcoplasmic/endoplasmic reticulum Ca2+ pump (SERCA)2a or SERCA2b cDNA. Ca2+ uptake by microsomes from transfected cells revealed that the Ca2+ affinity of the SERCA2b Ca2+ pump (K0.5 0.17 +/- 0.01-mu-M) was higher than that of the SERCA2a Ca2+ pump (K0.5 0.31 +/- 0.02-mu-m). Thapsigargin-sensitivity was found to be identical for the two isoforms. The Ca2+ affinity of both the SERCA2a and SERCA2b Ca2+ pumps was decreased by a factor of two when they were co-expressed with phospholamban.

引用

页码：591 / 595

页数：5

共 34 条

[1] BRANDL CJ, 1987, J BIOL CHEM, V262, P3768
[2] BURK SE, 1989, J BIOL CHEM, V264, P18561
[3] COONS AH, 1950, J EXP MED, V91, P1, DOI 10.1084/jem.91.1.1
[4] CULLEN BR, 1987, METHOD ENZYMOL, V152, P684
[5] DAVIS BA, 1983, J BIOL CHEM, V258, P3587
[6] DESMEDT H, 1991, J BIOL CHEM, V266, P7092
[7] EXPRESSION OF ENDOPLASMIC-RETICULUM CA-2+-PUMP ISOFORMS AND OF PHOSPHOLAMBAN IN PIG SMOOTH-MUSCLE TISSUES
EGGERMONT, JA
WUYTACK, F
VERBIST, J
CASTEELS, R
[J]. BIOCHEMICAL JOURNAL, 1990, 271 (03) : 649 - 653
[8] EVIDENCE FOR 2 ISOFORMS OF THE ENDOPLASMIC-RETICULUM CA-2+ PUMP IN PIG SMOOTH-MUSCLE
EGGERMONT, JA
WUYTACK, F
DEJAEGERE, S
NELLES, L
CASTEELS, R
[J]. BIOCHEMICAL JOURNAL, 1989, 260 (03) : 757 - 761
[9] FALCHETTO R, 1991, J BIOL CHEM, V266, P2930
[10] LOCALIZATION OF PHOSPHOLAMBAN IN SMOOTH-MUSCLE USING IMMUNOGOLD ELECTRON-MICROSCOPY
FERGUSON, DG
YOUNG, EF
RAEYMAEKERS, L
KRANIAS, EG
[J]. JOURNAL OF CELL BIOLOGY, 1988, 107 (02) : 555 - 562

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