IDENTIFICATION OF A 32-KDA ANTHER MARKER PROTEIN FOR ANDROGENIC RESPONSE IN MAIZE, ZEA-MAYS L

被引：15

作者：

VERGNE, P

RICCARDI, F

BECKERT, M

DUMAS, C

机构：

[1] INRA,AMELIORAT PLANTES STN,F-63039 CLERMONT FERRAND,FRANCE

[2] UNIV LYON 1,INRA 23879,CNRS,EP 20,F-69622 VILLEURBANNE,FRANCE

来源：

THEORETICAL AND APPLIED GENETICS | 1993年 / 86卷 / 07期

关键词：

MAIZE; MARKER PROTEIN; COLD PRETREATMENT; ANDROGENESIS; EMBRYOGENESIS;

D O I：

10.1007/BF00212611

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Variations in the whole anther protein pattern have been investigated in a highly androgenic maize hybrid during the inductive pretreatment for androgenesis. It was found that a 32-kDa protein (MAR32) is induced and accumulates in the anthers during cold pretreatment of the tassel. A positive correlation between the rate of embryo formation via anther culture and the level of this protein after 7 days of cold treatment was observed. In addition, the in vivo synthesis of this protein by cold-pretreated anthers was demonstrated. Different responsive and non-responsive genotypes were also evaluated, and the accumulation of MAR32-like protein was only observed in certain responsive genotypes. The results suggest that the protein MAR32 is a marker for a form of androgenic responsiveness in maize.

引用

页码：843 / 850

页数：8

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