OPEN READING FRAMES FLANKING THE PEPTIDYL-TRANSFER-RNA HYDROLASE-ENCODING GENE OF ESCHERICHIA-COLI

被引：3

作者：

GALINDO, JM ^{[1
]}

GUARNEROS, G ^{[1
]}

DELAVEGA, FM ^{[1
]}

机构：

[1] INST POLITECN NACL,CTR INVEST & ESTUDIOS AVANZADOS,DEPT MOLEC BIOL & GENET,MEXICO CITY 07000,DF,MEXICO

来源：

GENE | 1994年 / 151卷 / 1-2期

关键词：

GTP-BINDING MOTIF; PTH GENE; CAP-BINDING SITE; PRS GENE; SEQUENCING; MAXICELL; BACTERIOPHAGE-LAMBDA; TRANSLATION;

D O I：

10.1016/0378-1119(94)90647-5

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The nucleotide (nt) sequences flanking the peptidyl-tRNA hydrolase-encoding gene (pth) of Escherichia coli were determined and analyzed. A coding open reading frame (ORF-3), identified just downstream from pth, had a deduced amino acid (aa) sequence homologous to a family of GTP-binding proteins. We found discrepancies between the ORF-3 sequence from a plasmid clone used in previous studies and another one derived from Kohara's phage collection. Two putative genes, ORF-4 and ORF-2, were also found upstream from pth. ORF-4 could code for a 393-aa peptide homologous to membrane-bound proteins. The nt sequence between ORF-2 and pth revealed the existence of a CAP-binding site correctly positioned to regulate the expression of ORF-2.

引用

页码：153 / 156

页数：4

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