CARBONIC-ANHYDRASE ACTIVITY IN MYELIN FRACTIONS FROM RAT OPTIC NERVES

被引：8

作者：

FAGG, GE ^{[1
]}

LANE, JD ^{[1
]}

NEUHOFF, V ^{[1
]}

WAEHNELDT, TV ^{[1
]}

机构：

[1] MAX PLANCK INST EXPTL MED,FORSCHUNGSSTELLE NEUROCHEM,D-3400 GOTTINGEN,FED REP GER

来源：

NEUROSCIENCE LETTERS | 1979年 / 12卷 / 2-3期

关键词：

D O I：

10.1016/0304-3940(79)96065-8

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The carbonic anhydrase activity of myelin fractions isolated from the optic nerves of adult and immature (20-day-old) rats was examined. The specific activity in both total homogenate and myelin fractions was about 2-fold higher in adult than in immature animals and at both ages, the activity in the homogenate was higher than in myelin. After subfractionation by zonal gradient centrifugation, it was shown that carbonic anhydrase activity was greatest in the heaviest myelin particles at both ages. These data are consistent with the hypothesis that a small proportion of the total enzyme activity is localised in myelin. © 1979.

引用

页码：219 / 222

页数：4

共 11 条

[1] QUANTITATION OF MYELIN CARBONIC-ANHYDRASE - DEVELOPMENT AND SUBFRACTIONATION OF RAT-BRAIN MYELIN AND COMPARISON WITH MYELIN FROM OTHER SPECIES [J].

CAMMER, W ;

BIELER, L ;

FREDMAN, T ;

NORTON, WT .

BRAIN RESEARCH, 1977, 138 (01) :17-28

[2]

FAGG GE, BRAIN RES

[3]

LOWRY OH, 1951, J BIOL CHEM, V193, P265

[4] CARBONIC ANHYDRASE - CHEMISTRY PHYSIOLOGY AND INHIBITION [J].

MAREN, TH .

PHYSIOLOGICAL REVIEWS, 1967, 47 (04) :595-+

[5]

Norton W.T., 1977, MYELIN, P161

[6] MYELINATION IN RAT-BRAIN - METHOD OF MYELIN ISOLATION [J].

NORTON, WT ;

PODUSLO, SE .

JOURNAL OF NEUROCHEMISTRY, 1973, 21 (04) :749-757

[7] SOLUBLE AND MEMBRANE-BOUND CARBONIC-ANHYDRASES FROM RAT CNS - REGIONAL-DEVELOPMENT [J].

SAPIRSTEIN, VS ;

LEES, MB ;

TRACHTENBERG, MC .

JOURNAL OF NEUROCHEMISTRY, 1978, 31 (01) :283-287

[8] DENSITY AND PROTEIN PROFILES OF MYELIN FROM 2 REGIONS OF YOUNG AND ADULT RAT CNS [J].

WAEHNELDT, TV .

BRAIN RESEARCH BULLETIN, 1978, 3 (01) :37-44

[9] ONTOGENETIC STUDY OF A MYELIN-DERIVED FRACTION WITH 2'-3'-CYCLIC NUCLEOTIDE 3'-PHOSPHOHYDROLASE ACTIVITY HIGHER THAN THAT OF MYELIN [J].

WAEHNELDT, TV .

BIOCHEMICAL JOURNAL, 1975, 151 (02) :435-&

[10]

WILBUR KM, 1948, J BIOL CHEM, V176, P147

← 1 2 →