SILVER STAINING OF SYNAPTONEMAL COMPLEXES IN SURFACE SPREADS FOR LIGHT AND ELECTRON-MICROSCOPY

被引：104

作者：

DRESSER, ME

MOSES, MJ

机构：

[1] Department of Anatomy, Duke University Medical Center, Durham

来源：

EXPERIMENTAL CELL RESEARCH | 1979年 / 121卷 / 02期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/0014-4827(79)90023-5

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Synaptonemal complexes (SCs), axes of the X and Y chromosomes, and nucleoli in surface-spread preparations of spermatocytes are selectively stained for both light and electron microscopy by ammoniacal silver. Combined with a simple technique for transferring material from glass slides to grids, sequential light and electron microscopic analysis of full SC complements is possible with no further preparation. This new method has potential for both basic and clinical cytogenetic research. © 1979.

引用

页码：416 / 419

页数：4

共 14 条

[1] BRINKLEY BR, 1964, J CELL BIOL, V23, pA14
[2] LOCALIZATION OF SYNAPTONEMAL COMPLEX UNDER LIGHT-MICROSCOPE
ESPONDA, P
STOCKERT, JC
[J]. CHROMOSOMA, 1978, 68 (01) : 83 - 90
[3] FELLUGA B, 1976, J SUBMICR CYTOL PATH, V8, P347
[4] SYNAPTONEMAL COMPLEX AND CHROMOSOME STRUCTURE
GILLIES, CB
[J]. ANNUAL REVIEW OF GENETICS, 1975, 9 : 91 - 105
[5] VISUALIZATION OF NUCLEOLAR ORGANIZER REGIONS IN MAMMALIAN CHROMOSOMES USING SILVER STAINING
GOODPASTURE, C
BLOOM, SE
[J]. CHROMOSOMA, 1975, 53 (01) : 37 - 50
[6] ADHESION OF CELLS TO SURFACES COATED WITH POLYLYSINE - APPLICATIONS TO ELECTRON-MICROSCOPY
MAZIA, D
SCHATTEN, G
SALE, W
[J]. JOURNAL OF CELL BIOLOGY, 1975, 66 (01) : 198 - 200
[7] SYNAPTONEMAL COMPLEX KARYOTYPING IN SPERMATOCYTES OF CHINESE-HAMSTER (CRICETULUS-GRISEUS) .1. MORPHOLOGY OF AUTOSOMAL COMPLEMENT IN SPREAD PREPARATIONS
MOSES, MJ
[J]. CHROMOSOMA, 1977, 60 (02) : 99 - 125
[8] SYNAPTONEMAL COMPLEX KARYOTYPING IN SPERMATOCYTES OF CHINESE-HAMSTER (CRICETULUS-GRISEUS) .2. MORPHOLOGY OF XY PAIR IN SPREAD PREPARATIONS
MOSES, MJ
[J]. CHROMOSOMA, 1977, 60 (02) : 127 - 137
[9] MOSES MJ, 1977, CHROMOSOMES TODAY, V6, P71
[10] MOSES MJ, 1977, MOL HUMAN CYTOGENETI, V7, P101

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