STERILE HOST YEASTS (SHY) - EUKARYOTIC SYSTEM OF BIOLOGICAL CONTAINMENT FOR RECOMBINANT DNA EXPERIMENTS

被引：819

作者：

BOTSTEIN, D ^{[1
]}

FALCO, SC ^{[1
]}

STEWART, SE ^{[1
]}

BRENNAN, M ^{[1
]}

SCHERER, S ^{[1
]}

STINCHCOMB, DT ^{[1
]}

STRUHL, K ^{[1
]}

DAVIS, RW ^{[1
]}

机构：

[1] STANFORD UNIV,SCH MED,DEPT BIOCHEM,STANFORD,CA 94305

来源：

GENE | 1979年 / 8卷 / 01期

基金：

美国国家科学基金会;

关键词：

2-μ; plasmid; HV2; nonmating mutants; pBR322 plasmid vectors; Saccharomyces cerevisiae;

D O I：

10.1016/0378-1119(79)90004-0

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A system of biological containment for recombinant DNA experiments in Saccharomyces cerevisiae (Brewer's/Baker's yeast) is described. The principle of containment is sterility: the haploid host strains all contain a matingtype-non-specific sterile mutation. The hosts also contain four auxotrophic mutations suitable for selection for the various kinds of vectors used. All vectors are derivatives of pBR322 which can be selected and maintained in both yeast and Escherichia coli. The system has recently been certified at the HV2 level by the National Institutes of Health. © 1979.

引用

页码：17 / 24

页数：8

共 11 条

[1] TRANSFORMATION OF YEAST BY A REPLICATING HYBRID PLASMID [J].