PARTICLE BOMBARDMENT-MEDIATED TRANSIENT EXPRESSION OF A BRAZIL NUT METHIONINE-RICH ALBUMIN IN BEAN (PHASEOLUS-VULGARIS L)

被引：33

作者：

ARAGAO, FJL ^{[1
]}

DESA, MFG ^{[1
]}

ALMEIDA, ER ^{[1
]}

GANDER, ES ^{[1
]}

RECH, EL ^{[1
]}

机构：

[1] EMPRESA BRASILEIRA PESQUISA AGROPECUARIA,CTR NACL PESQUISAS RECURSOS GENET & BIOTECNOL,POB 02372,BRASILIA,DF,BRAZIL

来源：

PLANT MOLECULAR BIOLOGY | 1992年 / 20卷 / 02期

关键词：

BRAZIL NUT; 2S ALBUMIN GENE; GENE TRANSFER; PHASEOLUS-VULGARIS; PARTICLE BOMBARDMENT;

D O I：

10.1007/BF00014508

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Bean (Phaseolus vulgaris L.) mature embryos were transformed using biolistic methods with a plasmid containing 2S albumin and beta-glucuronidase structural sequences, both under the control of the 35S CaMV promoter. We have shown that chimaeric tissues could be obtained and that both structural sequences were expressed to similar levels.

引用

页码：357 / 359

页数：3

共 6 条

[1] DESA MFG, 1988, J CELL SCI, V89, P151
[2] GANDER ES, 1991, PLANT MOL BIOL, V16, P437, DOI 10.1007/BF00023994
[3] JEFFERSON RA, 1987, EMBO J, V6, P3901
[4] HIGH-VELOCITY MICROPROJECTILES FOR DELIVERING NUCLEIC-ACIDS INTO LIVING CELLS
KLEIN, TM
WOLF, ED
WU, R
SANFORD, JC
[J]. NATURE, 1987, 327 (6117) : 70 - 73
[5] A REVISED MEDIUM FOR RAPID GROWTH AND BIO ASSAYS WITH TOBACCO TISSUE CULTURES
MURASHIGE, T
SKOOG, F
[J]. PHYSIOLOGIA PLANTARUM, 1962, 15 (03) : 473 - 497
[6] RECH EL, 1991, IN PRESS TECHNIQUE

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