DIFFERENTIATION OF MUCOPLASMALIKE ORGANISMS (MLOS) IN EUROPEAN FRUIT-TREES BY PCR USING SPECIFIC PRIMERS DERIVED FROM THE SEQUENCE OF A CHROMOSOMAL FRAGMENT OF THE APPLE PROLIFERATION MLO

被引:73
作者
JARAUSCH, W
SAILLARD, C
DOSBA, F
BOVE, JM
机构
[1] INRA, RECH FRUITIERES STN, BORDEAUX, FRANCE
[2] INRA, BIOL CELLULAIRE & MOLEC LAB, BORDEAUX, FRANCE
关键词
D O I
10.1128/AEM.60.8.2916-2923.1994
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A 1.8-kb chromosomal DNA fragment of the mycoplasmalike organism (MLO) associated with apple proliferation was sequenced. Three putative open reading frames were observed on this fragment. The protein encoded by open reading frame 2 shows significant homologies with bacterial nitroreductases. From the nucleotide sequence four primer pairs for PCR were chosen to specifically amplify DNA from MLOs associated with European diseases of fruit trees. Primer pairs specific for (i) Malus-affecting MLOs, (ii) Malus- and Prunus-affecting MLOs, and (iii) Malus-, Prunus-, and Pyrus-affecting MLOs were obtained. Restriction enzyme analysis of the amplification products revealed restriction fragment length polymorphisms between Malus-, Prunus, and Pyrus-affecting MLOs as well as between different isolates of the apple proliferation MLO. No amplification with either primer pair could be obtained with DNA from 12 different MLOs experimentally maintained in periwinkle.
引用
收藏
页码:2916 / 2923
页数:8
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