NEGATIVE EFFECTS OF WILD-TYPE P53 AND S-MYC ON CELLULAR GROWTH AND TUMORIGENICITY OF GLIOMA-CELLS - IMPLICATION OF THE TUMOR-SUPPRESSOR GENES FOR GENE-THERAPY

Human (U251, U87, U343) and rat glioma cell lines (C6, 9L) were examined by the reverse transcriptase polymerase chain reaction and subsequent nucleotide sequencing analysis to see whether they express wild type (wt)-p53 or mutated form (mut)-p53 messages. Results showed that U87, U343, and C6 cells expressed wt-p53 messages whereas U251 and 9L cells expressed mut-p53 messages. All these cell lines were transfected with wt-p53 cDNA or the s-myc gene linked to the mouse mammary tumor virus (MMTV) promoter. Of several G418-resistant clones obtained from each transfection, a few expressed the s-Myc or wt-p53 proteins. Independent of mutations in the intrinsic p53 gene, the cellular growth in vitro and tumorigenicity in nude mice of these clones were drastically suppressed, the extent of suppression being correlated with the expression level of the transfected gene. Flow-cytometric analysis demonstrated that both p53 and s-Myc arrested the cell cycle at the G1/S boundary. These data suggest that these genes having negative effects on tumor eel proliferation could be used in gene therapy of gliomas, which are caused by alteration of the p53 gene or by some other genetic change.