CONSECUTIVE DEMONSTRATION OF CATECHOLAMINES AND DOPAMINE-BETA-HYDROXYLASE WITHIN THE SAME SPECIMEN

被引:31
作者
KONIG, R
机构
[1] Anatomisches Institut der Universität, Heidelberg, D-6900
关键词
D O I
10.1007/BF00508451
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
A combined method was developed for characterization and differentiation of catecholamines in neuron populations containing more than one catecholamine, e.g. dopamine and norepinephrine. Its application to small intensely fluorescent (SIF-) cell clusters in sympathetic ganglia allows the successive demonstration of glyoxylic acid-induced catecholamine fluorescence and dopamine-β-hydroxylase by indirect immunofluorescence within the same tissue section. Applying this technique to an example, two types of SIF-cells were demonstrated in the guinea pig superior cervical ganglion. © 1979 Springer-Verlag.
引用
收藏
页码:301 / 305
页数:5
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