IMMUNOELECTRON MICROSCOPY OF ROSENTHAL FIBERS

被引:29
作者
LACH, B
SIKORSKA, M
RIPPSTEIN, P
GREGOR, A
STAINES, W
DAVIE, TR
机构
[1] UNIV OTTAWA,DEPT PATHOL,OTTAWA K1Y 4E9,ONTARIO,CANADA
[2] UNIV OTTAWA,DEPT ANAT,OTTAWA K1Y 4E9,ONTARIO,CANADA
[3] NATL RES COUNCIL CANADA,DIV BIOL SCI,OTTAWA K1A 0R6,ONTARIO,CANADA
[4] UNIV MANITOBA,DEPT BIOCHEM,WINNIPEG R3T 2N2,MANITOBA,CANADA
关键词
ROSENTHAL FIBERS; UBIQUITIN; IMMUNOHISTOCHEMISTRY; GLIAL FIBRILLARY ACIDIC PROTEIN; VIMENTIN;
D O I
10.1007/BF00310130
中图分类号
R74 [神经病学与精神病学];
学科分类号
摘要
Seventeen intracerebral gliomas containing Rosenthal fibers (RF) were studied by an immunoperoxidase method for localization of ubiquitin (UB), glial fibrillary acidic protein (GFAP), desmin and vimentin (VIM). The majority of RF showed an immunohisto-chemically negative core surrounded by a ring of overlapping reactions for UB, GFAP and VIM. Many RF were entirely negative for UB and intermediate filaments (IF). Immunoelectron microscopic localization of UB and GFAP was performed on seven selected tumors. UB was found in all RF and on IF in the proximity of RF. GFAP reaction was localized on astrocytic IF, including those trapped within RF, and within the granular component of some RF. In contrast to the light microscopic studies, neither GFAP-nor UB-negative RF were found on immunoelectron microscopy. VIM reaction on IF and a few RF was demonstrated in one tumor processed at low temperature into Lowicryl; it was much weaker than that for GFAP. Many cells with RF contained lysosome-like inclusions with material displaying electron density similar to adjacent RF; few of these inclusions were reactive for UB. It is concluded that RF formation is associated with ubiquitination of astrocytic IF. GFAP- and VIM-immunoreactive IF and products of their disintegration contribute to RF material. It is also suggested that the lysosomal system of astrocytes partially degrades RF.
引用
收藏
页码:503 / 509
页数:7
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