AN IMPROVED DIRECT VIABLE COUNT FOR THE ENUMERATION OF BACTERIA IN MILK

被引：14

作者：

BUCHRIESER, C ^{[1
]}

KASPAR, CW ^{[1
]}

机构：

[1] UNIV WISCONSIN,FOOD RES INST,MADISON,WI 53706

来源：

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY | 1993年 / 20卷 / 04期

关键词：

DIRECT VIABLE COUNT; CIPROFLOXACIN; RAPID METHOD;

D O I：

10.1016/0168-1605(93)90167-F

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

The direct viable count (DVC), a microscopic method for the enumeration of Viable bacteria, was modified by replacing nalidixic acid with ciprofloxacin. This modification made it possible to apply this method to a variety of Gram-negative and Gram-positive bacteria which was not previously possible. Of the four antibiotics tested (nalidixic acid, novobiocin, ciprofloxacin and mitomycin C), ciprofloxacin and mitomycin C were the only ones effective for use in the DVC with all of the bacteria tested. In addition, ciprofloxacin could be used at a single concentration (1 mu g/ml) while adjustments were necessary with the other antibiotics when examining bacteria from different genera and, in some instances, from different species. The use of ciprofloxacin in the DVC resulted in viable cells that had elongated by 5-11 times their original length. We conclude that the modified DVC will be useful in growth and survival studies of bacterial pathogens and spoilage organisms in milk and other foods.

引用

页码：227 / 237

页数：11

共 21 条

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