THE GENE ENCODING TOPOISOMERASE-II FROM PLASMODIUM-FALCIPARUM

被引:38
作者
CHEESMAN, S [1 ]
MCALEESE, S [1 ]
GOMAN, M [1 ]
JOHNSON, D [1 ]
HORROCKS, P [1 ]
RIDLEY, RG [1 ]
KILBEY, BJ [1 ]
机构
[1] UNIV EDINBURGH,INST CELL & MOLEC BIOL,EDINBURGH EH9 3JR,MIDLOTHIAN,SCOTLAND
基金
英国医学研究理事会;
关键词
D O I
10.1093/nar/22.13.2547
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The gene for topoisomerase II has been isolated from genomic libraries of strain K1 of the human malarial parasite, Plasmodium falciparum. The sequence reveals an open reading frame of 4194 nucleotides which predicts a polypeptide of 1398 amino acids. There are apparently no introns. The sequence is present as a single copy which has an identity of 47.4% and a similarity of 65.4% with its human homologue. Sequences conserved in topoisomerase II from other species are present in Pftopoisomerase II but in addition it has two adjacent asparagine-rich insertions which are unique to it. We have also detected asparagine-rich regions in the gene for PfDNA polymerase alpha. The gene for Pftopoisomerase II has been localised to chromosome 14 and northern analysis reveals a transcript of 5.8kb. Two independent antisera raised;in mice against glutathione-S-transferase fusion proteins containing the amino terminal portion of the malarial protein detect a weak band on western blots at about 160kDa, the expected size of the protein. Use of the same antisera for immunofluorescence analysis suggests that the protein is present at all stages of intraerythrocytic growth of the parasite.
引用
收藏
页码:2547 / 2551
页数:5
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