UBIQUITINATION OF THE RAT UTERINE ESTROGEN-RECEPTOR - DEPENDENCE ON ESTRADIOL

被引：77

作者：

NIRMALA, PB ^{[1
]}

THAMPAN, RV ^{[1
]}

机构：

[1] UNIV HYDERABAD,SCH LIFE SCI,DEPT BIOCHEM,HYDERABAD 500046,ANDHRA PRADESH,INDIA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 213卷 / 01期

关键词：

D O I：

10.1006/bbrc.1995.2093

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Using polyclonal antibodies against estrogen receptor and ubiquitin, the ubiquitination of the estrogen receptor has been demonstrated in both in vitro and in vivo conditions. The ubiquitination of the estrogen receptor is estradiol specific and is enhanced by estradiol. Estrogen withdrawal is associated with decreased ubiquitination of the estrogen receptor. (C) 1995 Academic Press, Inc.

引用

页码：24 / 31

页数：8

共 19 条

[1] A RAPID, SENSITIVE METHOD FOR DETECTION OF ALKALINE-PHOSPHATASE CONJUGATED ANTI-ANTIBODY ON WESTERN BLOTS [J].

BLAKE, MS ;

JOHNSTON, KH ;

RUSSELLJONES, GJ ;

GOTSCHLICH, EC .

ANALYTICAL BIOCHEMISTRY, 1984, 136 (01) :175-179

[2] IMPROVED SILVER STAINING OF PLANT-PROTEINS, RNA AND DNA IN POLYACRYLAMIDE GELS [J].

BLUM, H ;

BEIER, H ;

GROSS, HJ .

ELECTROPHORESIS, 1987, 8 (02) :93-99

[3] ATP-DEPENDENT CONJUGATION OF RETICULOCYTE PROTEINS WITH THE POLYPEPTIDE REQUIRED FOR PROTEIN-DEGRADATION [J].

CIECHANOVER, A ;

HELLER, H ;

ELIAS, S ;

HAAS, AL ;

HERSHKO, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1980, 77 (03) :1365-1368

[4] THE UBIQUITIN SYSTEM - FUNCTIONS AND MECHANISMS [J].

FINLEY, D ;

VARSHAVSKY, A .

TRENDS IN BIOCHEMICAL SCIENCES, 1985, 10 (09) :343-347

[5]

GLOTZER M, 1991, NATURE, V349, P132, DOI 10.1038/349132a0

[6]

HERSHKO A, 1971, J BIOL CHEM, V246, P710

[7] PROPOSED ROLE OF ATP IN PROTEIN BREAKDOWN - CONJUGATION OF PROTEINS WITH MULTIPLE CHAINS OF THE POLYPEPTIDE OF ATP-DEPENDENT PROTEOLYSIS [J].

HERSHKO, A ;

CIECHANOVER, A ;

HELLER, H ;

HAAS, AL ;

ROSE, IA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1980, 77 (04) :1783-1786

[8] MECHANISMS OF INTRACELLULAR PROTEIN BREAKDOWN [J].

HERSHKO, A ;

CIECHANOVER, A .

ANNUAL REVIEW OF BIOCHEMISTRY, 1982, 51 :335-364

[9] THE SHORT-LIVED MAT-ALPHA-2 TRANSCRIPTIONAL REGULATOR IS UBIQUITINATED INVIVO [J].

HOCHSTRASSER, M ;

ELLISON, MJ ;

CHAU, V ;

VARSHAVSKY, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (11) :4606-4610

[10]

HORWITZ KB, 1983, J BIOL CHEM, V258, P7603

← 1 2 →