BIOSYNTHESIS OF A 7-ALPHA-METHOXYCEPHALOSPORIN - INCORPORATION OF MOLECULAR-OXYGEN

被引：36

作者：

OSULLIVAN, J

APLIN, RT

STEVENS, CM

ABRAHAM, EP

机构：

[1] UNIV OXFORD, SIR WILLIAM DUNN SCH PATHOL, OXFORD OX1 3RE, ENGLAND

[2] UNIV OXFORD, DYSON PERRINS LAB, OXFORD OX1 3QY, ENGLAND

来源：

BIOCHEMICAL JOURNAL | 1979年 / 179卷 / 01期

关键词：

D O I：

10.1042/bj1790047

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A 7-.alpha.-methoxycephalosporin containing a carbamoyloxymethyl substituent at C-3 (cephamycin C) was isolated from the extracellular fluid of an aqueous suspension of Streptomyces clavuligerus shaken in the presence of 18O2. The cephalosporin was converted into its N-acetyl dimethyl ester and the distribution of 18O in the latter determined by chemical-ionization mass spectrometry. The results indicate that the O atom of the methoxy group, and that linked to the exocyclic methylene group at C-3, is derived from molecular O2.

引用

页码：47 / 52

页数：6

共 19 条

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